Mycobacterium tuberculosis rpoB gene DNA sequencing: implications for detection of rifamycin resistance
نویسندگان
چکیده
منابع مشابه
Contribution of rpoB mutations to development of rifamycin cross-resistance in Mycobacterium tuberculosis.
The contributions of 23 insertion, deletion, or missense mutations within an 81-bp fragment of rpoB, the gene encoding the beta-subunit of the DNA-dependent RNA polymerase of Mycobacterium tuberculosis, to the development of resistance to rifamycins (rifampin, rifabutin, rifapentine, and KRM-1648) in 29 rifampin-resistant clinical isolates were defined. Specific mutant rpoB alleles led to the d...
متن کاملRapid detection of rpoB gene mutations conferring rifampin resistance in Mycobacterium tuberculosis.
Multidrug-resistant Mycobacterium tuberculosis strains are widespread and present a challenge to effective treatment of this infection. The need for a low-cost and rapid detection method for clinically relevant mutations in Mycobacterium tuberculosis that confer multidrug resistance is urgent, particularly for developing countries. We report here a novel test that detects the majority of clinic...
متن کاملMutations in the RpoB Gene of Multidrug-Resistant Mycobacterium Tuberculosis Isolates from Semarang, Indonesia
ABSTRACTTuberculosis is an infectious disease which occurs widely in the world and becomes one of the top 10 causes of death worldwide. Mutations of the RpoB gene cause the resistance of Mycobacterium tuberculosis to rifampicin that contributes to the occurrence of MDR-TB. This study aimed to determine the pattern of rpoB gene polymorphisms in the MDR M. tuberculosis in Semarang, Indonesia. Mos...
متن کاملDetection of rpoB mutations in Mycobacterium tuberculosis with LightCycler technology.
In a recent article, Edwards et al. evaluated three biprobes to detect mutations in rifampin-resistant isolates of Mycobacterium tuberculosis (1). The authors claimed that their approach allowed the detection of all mutations in the 46 isolates studied. We read their paper with interest, because we have used LightCycler PCR to detect resistance to rifampin and isoniazid in M. tuberculosis (3). ...
متن کاملCharacterization of Mutations in the Rpob and Katg Gene of Mycobacterium Tuberculosis Isolates From Pasteur Institute of Tehran
Objective: The Rifampicin resistance and susceptibility of Mycobacterium tuberculosis are caused by mutations in the 81-base pair region of the rpoB gene encoding the b-subunit of RNA polymerase. Methods: Isoniazid resistance of M. tuberculosis is related to mutations in inha , oxyR and ahpC genes which 30 to 90 percent of Isoniazid resistance is occurred in 3015 codons of kat...
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ژورنال
عنوان ژورنال: Journal of Antimicrobial Chemotherapy
سال: 1999
ISSN: 1460-2091,0305-7453
DOI: 10.1093/jac/44.2.294